In vitro and in vivo evaluation of genotoxicity, cytotoxicity, and protective effects of synthetic chalcones (E)-3-(4-chlorophenyl)-1-phenyl-2-propen-1-one (4-CL) and (E)-3-(3,4-dimethoxyphenyl)-1- phenyl-2-propen-1-one (DMF)

The chalcones (E)-3-(4-chlorophenyl)-1-phenyl-2-propen-1-one (4-CL) and (E)-3-(3,4-dimethoxyphenyl)-1-phenyl-2 -propen-1-one (DMF) are versatile easily synthesized into low-cost compounds that have a wide spectrum of biological activities. In this study, the cytotoxic, genotoxic modulatory activities 4-CL DMF were evaluated using Ames test mouse micronucleus assay. results revealed both did not show mutagenic activity in Salmonella typhimurium strains TA98 TA100, demonstrated significant antimutagenicity (p< 0.05) when co-administered with sodium azide (SA) strain TA100. assay, showed increase frequency micronucleated polychromatic erythrocytes (MNPCE) at 24 h 48 h, revealing effect. co-treatment mitomycin C (MMC) there was decrease MNPCE 24h less concentrated dose 48h, demonstrating its antigenotoxic activity. polychromatic/ normochromatic erythrocyte (PCE/ NCE) ratio 0.05), indicating cytotoxicity. However, MMC PCE/NCE within hours, anticytotoxicity. Furthermore, biphasic dose-response behavior observed chalcones, co-administration SA, Test MMC, hours exposure, genotoxic, antigenotoxic, anticytotoxic no properties.